Structural View of the Ran–Importin β Interaction at 2.3 Å Resolution
نویسندگان
چکیده
D-44227 Dortmund nucleus (Ohtsubo et al., 1989; Bischoff and Ponstingl, Germany 1991a; Renault et al., 1998). This differential localization †Zentrum für Molekulare Biologie of the regulators of Ran’s nucleotide-bound state should Universität Heidelberg thus result in a steep RanGTP gradient across the nuD-69120 Heidelberg clear envelope with a high concentration in the nucleus Germany and very low levels in the cytoplasm. Transport recep-
منابع مشابه
Importazole, a small molecule inhibitor of the transport receptor importin-β.
During interphase, the transport receptor importin-β carries cargoes into the nucleus, where RanGTP releases them. A similar mechanism operates in mitosis to generate a gradient of active spindle assembly factors around mitotic chromosomes. Importin-β and RanGTP have been implicated in additional cellular processes, but the precise roles of the Ran/importin-β pathway throughout the cell cycle r...
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The translocation of macromolecules into the nucleus is a fundamental eukaryotic process, regulating gene expression, cell division and differentiation, but which is impaired in a range of significant diseases including cancer and viral infection. The import of proteins into the nucleus is generally initiated by a specific, high affinity interaction between nuclear localisation signals (NLSs) a...
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Importin-β is the main vector for interphase nuclear protein import and plays roles after nuclear envelope breakdown. Here we show that importin-β regulates multiple aspects of mitosis via distinct domains that interact with different classes of proteins in human cells. The C-terminal region (which binds importin-α) inhibits mitotic spindle pole formation. The central region (harboring nucleopo...
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Import of proteins into the nucleus proceeds through nuclear pore complexes and is largely mediated by nuclear transport receptors of the importin β family that use direct RanGTP-binding to regulate the interaction with their cargoes. We investigated nuclear import of the linker histone H1 and found that two receptors, importin β (Impβ) and importin 7 (Imp7, RanBP7), play a critical role in thi...
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عنوان ژورنال:
- Cell
دوره 97 شماره
صفحات -
تاریخ انتشار 1999